Device

Part:BBa_K1210003:Design

Designed by: Harland Brandon   Group: iGEM13_Lethbridge   (2013-10-15)


PK401 tagged dual coding test construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1457
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI.rc site found at 1600


Design Notes

This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.


Source

This part was synthesized by Genewiz. It contains genomic sequence from IBV virus (PK401 pseudoknot; non-infectious sequence), and from IAPV virusb(IAPV overlapping ORFs; only 7 aa used of the overlapping genes to avoid producing anything harmful).

References