![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1210003:Design
Designed by: Harland Brandon Group: iGEM13_Lethbridge (2013-10-15)
PK401 tagged dual coding test construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1457
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 90
Illegal BsaI.rc site found at 1600
Design Notes
This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.
Source
This part was synthesized by Genewiz. It contains genomic sequence from IBV virus (PK401 pseudoknot; non-infectious sequence), and from IAPV virusb(IAPV overlapping ORFs; only 7 aa used of the overlapping genes to avoid producing anything harmful).